Typically, access is provided across an institutional network to a range of IP addresses. If you are a member of an institution with an active account, you may be able to access content in one of the following ways: Get help with access Institutional accessĪccess to content on Oxford Academic is often provided through institutional subscriptions and purchases. prunifolia and response to salt stress of the resulting polyploids, as reflected in leaf and root morphology, changes in Na + accumulation, antioxidant capacity and plant hormone levels. In summary, we developed a system for the induction of polyploidy in M. Under normal growth conditions, gibberellins (GAs) levels were much lower in polyploid leaves than in diploid leaves however, after salt treatment, polyploid leaves showed upregulation of essential GAs synthesis genes. The polyploid plants also had considerably higher ABA and jasmonic acid levels than diploid plants under salt stress.
During salt stress, the polyploid leaves and roots accumulated less Na +, showed upregulated expression of three salt overly sensitive (SOS) pathway genes, and produced fewer reactive oxygen species. The results of hydroponic salt treatment showed that polyploid plants were more salt tolerant and had greater photosynthetic efficiency, thicker leaf epidermis and palisade tissues, and shorter but denser root systems than diploids. prunifolia was to use 2% DMSO and 0.05% colchicine for 2 days for leaves or 0.02% colchicine for stem segments. We found the best way to induce polyploidy in M. prunifolia and identified the resulting polyploids by stomatal observations and flow cytometry. We used a combination of colchicine and dimethyl sulfoxide (DMSO) to induce chromosome doubling in M. prunifolia might improve its stress tolerance, particularly to high salinity. Because polyploid plants are often more tolerant to abiotic stress than diploids, we wondered whether polyploidy induction in M.
The apple rootstock Malus prunifolia (Willd.) Borkh.
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